实验动物科学 ›› 2026, Vol. 43 ›› Issue (3): 27-30.DOI: 10.3969/ j. issn.1006-6179.2026.03.005

• 论著 • 上一篇    下一篇

75%乙醇开封后30 d内对金黄色葡萄球菌灭菌效力的验证

  

  1. (1.传染病溯源预警与智能决策全国重点实验室,中国疾病预防控制中心(中国预防医学科学院),北京 102206) (2.中国疾病预防控制中心传染病所,北京 102206)
  • 收稿日期:2025-04-22 出版日期:2026-03-28 发布日期:2026-07-06
  • 通讯作者: 李晓燕(1977—),女,研究员,研究方向为生物安全、实验动物科学。E-mail:lixy@chinacdc.cn。 卢选成(1977—),男,研究员,研究方向为生物安全、实验动物科学。E-mail:luxc@chinacdc.cn。
  • 作者简介:师晓萌(1989—),女,研究方向为实验动物科学。E-mail:shixm1@chinacdc.cn。
  • 基金资助:
    国家重点研发计划(2024YFF0728803)。

Validation of Sterilization Efficacy of 75% Ethanol Against Staphylococcus aureus within 30 d after Opening

  1.  (1.National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases (NITFID),Chinese Center For Disease Control And Prevention(Chinese Academy of Preventive Medicine), Beijing 102206,China) (2. Institute for Communicable Disease Prevention and Control, Chinese Center for Disease Control and Prevention,Beijing 102206,China)
  • Received:2025-04-22 Online:2026-03-28 Published:2026-07-06

摘要: 目的 验证75%乙醇开封后,其灭菌效力随时间增加的变化,确定其使用有效期,确保其消毒效力。方法 75%乙醇开封后灌注于喷壶中,于实验动物屏障设施内常温放置。以金黄色葡萄球菌为测试菌株,通过菌悬液定 量杀菌实验法检测开封后不同时间段75%乙醇(0~30 d)的杀菌效果。结果 菌悬液定量杀菌实验结果显示,75% 乙醇开封后室温存放至第30天,其对金黄色葡萄球菌的杀灭对数值仍维持在5.0以上。结论 75%乙醇开封后于 喷壶中常温放置,30 d内对金黄色葡萄球菌杀菌效力未见显著降低,可作为其延长使用期限的佐证。

关键词: 75%乙醇, 金黄色葡萄球菌, 消毒效力

Abstract: Objective To validate the time-dependent changes in the sterilization efficacy of 75% ethanol after opening and to determine its safe use period to ensure disinfection effectiveness.Methods After opening, 75% ethanol was transferred into a spray bottle and placed in a laboratory animal barrier facility at room temperature. A quantitative suspension bactericidal test was performed using Staphylococcus aureus as the test strain to evaluate the bactericidal efficacy of 75% ethanol at different time intervals (0 30 d) after opening.Results The quantitative suspension bactericidal test demonstrated no significant decline in the bactericidal efficacy of 75% ethanol against Staphylococcus aureus after 30 d of room temperature storage. The log reduction value (LRV) remained above 5.0 throughout the testing period. Conclusion After being transferred to spray bottle and stored at room temperature, 75% ethanol shows no significant decline in its bactericidal efficacy against Staphylococcus aureus within 30 d, which can serve as evidence for extending its service life.

Key words: 75% ethanol, Staphylococcus aureus, disinfection efficacy 

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